Abstrat

In recent years, interest in the cutaneous biological effects of high-energy visible light (400-450 nm) has been growing. In this study, we explored the impact of blue light (BL) on the repair of pyrimidine dimers, the main class of premutagenic DNA damage induced by sun exposure. We provide compelling evidence that exposure of in vitro reconstructed human epidermis to environmentally relevant doses of BL strongly decreases the rate of repair of cyclobutane pyrimidine dimers and pyrimidine (6-4) pyrimidone photoproducts induced by subsequent UVB irradiation. Using a highly sensitive and specific liquid chromatography-tandem mass spectrometry method, we did not observe the induction of pyrimidine dimers by BL alone. Finally, we showed that the application, during the BL exposure step, of a formulation containing a new filter called TriAsorB, which provides photoprotection against BL, prevented DNA repair. These results highlight the potentially harmful effects of BL on DNA repair and the importance of providing adequate skin protection against this wavelength range of sunlight.